VWR Hitachi Chromaster Ultra Rs
Ultra High-Performance Liquid Chromatography (UHPLC)
Visualize the future: high resolution, high sensitivity, and operational excellence
The VWR Hitachi ChromasterUltra Rs is an ultra high-performance liquid chromatography (UHPLC) system engineered for a new era of analytical demands. Designed to combine exceptional separation power, trace-level sensitivity, and high reliability, it delivers stable performance under extreme pressure while remaining intuitive to operate and easy to maintain.
With a maximum system pressure of 140 MPa (1400 bar) and a thoroughly minimized system volume, ChromasterUltra Rs enables both ultra high-speed and ultra high-resolution workflows. It also supports method transfer and routine work across UHPLC and standard HPLC ranges, providing long-term flexibility for evolving lab needs.
Key advantages
High resolution for complex samples
• 140 MPa (1400 bar) pressure capability enables demanding separations using long UHPLC columns and small particles.
• Designed to achieve industry-leading resolution with LaChromUltra II high-resolution columns, delivering 50,000+ theoretical plates per column, and enabling even higher resolution by connecting columns in series.
• Reduced piping length and low system volume improve gradient response and minimize extra-column dispersion—critical for high plate counts and sharp peak shapes.
High sensitivity without compromising resolution
• A total reflection capillary flow cell design in the diode array detector reduces dispersion and supports
high-sensitivity detection.
• An optional high-sensitivity flow cell (65 mm optical path length) further boosts sensitivity, enabling reliable profiling of trace components and impurities.
• The binary pump’s double corkscrew mixer promotes efficient solvent mixing in a low volume, supporting a highly stable baseline for gradient analysis.
Ease of use and lab efficiency
• Front-access maintenance supports day-to-day servicing and consumable replacement with minimal down
time.
• Wakeup/Sleep programs (via optional touchscreen GUI) automate pre- and post-analysis tasks.
• Practical built-in features improve workflow and safety, such as the built-in 3-liter waste tank integrated into the column oven and a large autosampler window with internal illumination for easy visual monitoring.
Low carryover for trace analysis
• The autosampler design targets extremely low carryover through:
– reduced injection-port dead volume,
– dedicated high-flow washing via a washing pump,
– two-solvent washing for needle inner wall and injection valve cleaning,
– and a back-flush function for the injection port.
System architecture (typical UHPLC configuration)
A ChromasterUltra Rs system is typically configured with:
• 6170 Binary Pump (high-pressure gradient)
• 6270 Autosampler with cooling/thermostat functionality
• 6310 Column Oven (Peltier cooling/heating)
• Detector options, such as:
– 6430 Diode Array Detector (DAD)
– 6420 UV-VIS Detector
– 6440 Fluorescence Detector (optional)
• Organizer (including interface control board)
• Optional GUI Controller (touchscreen)
Technical specifications (core modules)
6170 Binary Pump
• Gradient type: Binary high-pressure gradient
• Mechanism: Built-in two double plunger pumps (binary)
• Maximum pressure: 1400 bar (140 MPa)
• System delay volume: 120 μL (pump + 55 μL mixer + autosampler + 40 μL injection loop + detector)
• Mixer volume: 55 μL (double corkscrew mixer); two mixers supplied as standard. An additional double corkscrew mixer can be added for a total of 110 μL.
• Settable flow-rate range:
– 0.001–2.000 mL/min up to 1400 bar (140 MPa)
– 0.001–2.500 mL/min up to 800 bar (80 MPa)
– 0.001–5.000 mL/min (purge)
• Flow-rate accuracy: 10 μL/min or ±1.0% (0.200–2.500 mL/min, water)
• Flow-rate precision: SD 0.005 min or RSD 0.06% (1 mL/min)
• Pressure pulsation: <1% or 0.5 MPa (water)
• Composition precision: 0.15% RSD or SD 0.01 min (water / water + caffeine)
• Composition accuracy: ±0.5% (5–95%) (1 mL/min, water / water + caffeine)
• Composition time setting: 0.01 min
• Composition rate setting: 0.10%
• Solvent selector: Standard
• Eluent number: 4 total (2 for each pump)
• Degassing unit: 6 channels (standard; includes 2 channels for autosampler wash)
• Seal wash mechanism: Standard
• Auto purge valve: Standard
• Composition ripple: ≤0.1 mAU (55 μL mixer, DAD; specified solvent/flow/ratio conditions)
6270 Autosampler
• Maximum pressure: 1400 bar (140 MPa)
• Injection volume (cut injection):
– 0.1–20 μL (standard)
– 0.1–50 μL (optional)
– 0.1–500 μL (optional)
• Injection precision:
– Cut injection: ≤0.15% RSD (10 μL), ≤0.5% RSD (1 μL)
– All-volume injection: ≤0.7% RSD (1 μL)
• Carryover: <0.001% (sample: caffeine)
• Sample capacity options:
– 120 × 1.5 mL
– 195 × 1.0 mL (option)
– 72 × 4.0 mL (option)
– 2 × microtiter plates (96, 384) (option)
• Injection accuracy: ±2% (20 μL, n = 25)
• Linearity: ≥ 0.999
• Minimum injection operating time: 25 s (defined condition: time between autosampler start and injection valve switching)
• Washing function:
– Two-liquid washing for injection-port back flush
– Two-solvent washing for needle inner surface
– One solvent for needle outer surface
• Thermo function: Standard, 1–45 °C
• Automatic pre-/post-analysis tasks: Programmable via touchscreen GUI (optional)
• Loop offline function: Standard
6310 Column Oven
• Control system: Peltier block cooling/heating with air circulation
• Temperature setting range: 4–90 °C
• Temperature accuracy (with calibration):
– ±0.5 °C (20–50 °C)
– ±1.0 °C (50–90 °C)
• Temperature precision: ±0.1 °C (20–90 °C)
• Warm-up / cool-down time:
– 4 min from ambient (25 °C) to 40 °C
– 9 min from 40 °C to 20 °C at ambient (25 °C)
• Column capacity (without switching valve):
– 3 × 250 mm columns (max 1400 bar, using MEM column fitting)
– 3 × 300 mm columns (with PEEK fitting)
• Optional valve: 2-position / 6-port valve
• Easy column setting: Tool-less universal fitting
• MEM column fitting: Moment-Enhancing Mechanism (MEM), max pressure 1400 bar (140 MPa)
6430 Diode Array Detector (DAD)
• Light source: D2 (Hg for automatic wavelength check)
• Wavelength range: 190–650 nm
• Detector type: 1,024-bit
• Wavelength accuracy: ±1 nm
• Spectral bandwidth: 1 / 2 / 4 / 8 / 16 nm
• Flow cell (metric volume):
– Total reflection flow cell 2.2 μL (standard), 10 mm
– 14 μL (optional), 65 mm
• Noise: ≤ 0.5 × 10⁻⁵ AU (4 nm, TC = 2 s at 250 nm; reference method, specified conditions)
• Drift: ≤ 0.4 × 10⁻³ AU/hr (at 250 nm; environment within 2 °C/h; reference method, specified conditions)
• Linearity: 2.0 AU (5%)
• Data rate: 100 Hz (10 ms)
• Cell maximum pressure: 60 bar (6 MPa)
Columns for UHPLC and method flexibility
LaChromUltra II high-resolution columns (1.9 μm)
ChromasterUltra Rs is designed to fully utilize UHPLC column performance under very high pressure. LaChromUltra II C18 (1.9 μm) columns deliver high resolution and robustness for advanced separations, including challenging impurity profiling and complex mixtures.
Key capabilities highlighted for this column family:
• High system pressure tolerance up to 140 MPa
• Mobile phase flexibility across a broad pH range (pH 1 to 12)
• Ultra-high resolution by connecting columns in series, enabling very high plate counts
UHPLC-to-HPLC support
ChromasterUltra Rs supports a broad analytical range, enabling stable solvent delivery and reproducible gradient performance across both UHPLC and HPLC workflows—useful for development labs and QC environments that require method transfer, scalability, and continuity.
Built-in usability & reliability features
Stable delivery under high pressure
The binary pump is designed for reproducibility even under high pressure gradient conditions, supporting consistent results over a wide pressure range.
Operability that protects columns and reduces downtime
• Build-up flow system automatically accelerates/decelerates flow during start-up and flow changes, helping protect columns from rapid pressure/flow transitions.
• Solvent selector (standard) allows selection of different eluents per pump—useful for method development.
• 6-channel degassing (standard) helps prevent bubble-related issues during operation.
• Plunger washing unit supports automatic washing routines to reduce damage from salt precipitation and seal wear.
Practical system design
• Built-in 3-liter waste tank integrated in the column oven (improves safety and frees bench/floor space).
• Large autosampler viewing window with internal illumination to visually check operating status and samples during operation.
• Consumables and service points are accessible from the front for efficient routine maintenance.
Extensibility & system options
ChromasterUltra Rs offers a high degree of extensibility for a wide range of analytical needs, including:
• 2-column selector valve (2-position, 6-port) in the column oven for column switching and rapid condition screening.
• Column management system (optional) to track log information on analytical and guard columns (any manufacturer), with read/write via column oven connector or PC USB.
• Optional GUI controller (5.7″ color TFT touchscreen) to control all modules, build up to 10 programs (including timer, Wakeup, Sleep), and monitor consumables usage.
